CV

Education

• Ph.D. in Biophysics, University of Michigan, 2023 (expected)
• M.S. in Computational Medicine and Bioinformatics, 2023 (expected)
• M.S. in Biophysics, University of Michigan, 2020
• B.S. in Physics, Astronomy, and Astrophysics, The Ohio State University, 2018

Work experience

• 2018 to present: Graduate Student Research Assistant
  • University of Michigan Biophysics
  • Supervisor: Dawen Cai
• 2015 to 2018: Research Assistant
  • The Ohio State University Physics and Comprehensive Cancer Center
  • Supervisors: Pearlly Yan and Ralf Bundschuh

Publications

Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function

V. Saxena, J. Fitch, J. Ketz, P. White, A. Wetzel, M. A. Chanley, J. D. Spencer, B. Becknell, K. R. Pierce, S. W. Arregui, R. D. Nelson, G. J. Schwartz, V. Velazquez, L. A. Walker, X. Chen, P. Yan, D. S. Hains, and A. L. Schwaderer. Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function. Scientific Reports 9, 545 (2019).

ROR1-targeted delivery of miR-29b induces cell cycle arrest and therapeutic benefit in vivo in a CLL mouse model

C.-L. Chiang, S. Goswami, F. W. Frissora, Z. Xie, P. Yan, R. A. Bundschuh, L. A. Walker, X. Huang, R. Mani, X. Mo, S. Baskar, C. Rader, M. A. Phelps, G. Marcucci, J. C. Byrd, L. Lee, and N. Muthusamy. ROR1-targeted delivery of miR-29b induces cell cycle arrest and therapeutic benefit in vivo in CLL mouse model. Blood 134 (5) 432-444 (2019).

CLEAR: coverage-based limiting-cell experiment analysis for RNA-seq

L. A. Walker, M. G. Sovic, C.-L. Chiang, E. Hu, J. K. Denninger, X. Chen, E. D. Kirby, J. C. Byrd, N. Muthusamy, R. Bundschuh, and P. Yan. CLEAR: Coverage-based Limiting-cell Experiment Analysis for RNA-seq. Journal of Translational Medicine 18 (1), 63 (2020).

TraceMontage: A method for merging multiple independent neuronal traces

S. A. Dizaji, L. A. Walker, and D. Cai. TraceMontage: a Method for Merging Multiple Independent Neuronal Traces. Journal of Neuroscience Methods 332, 108560 (2020).

Light microscopy based approach for mapping connectivity with molecular specificity

F. Shen, M. Harrington, L. A. Walker, H. J. Chen, E. S. Boyden, and D. Cai. Light microscopy based approach for mapping connectivity with molecular specificity. Nature Communications 11, 4632 (2020).

Unsupervised Neural Tracing in Densely Labeled Multispectral Brainbow Images

B. Duan, L. A. Walker, D. Roossien, F. Shen, D. Cai, and Y. Yan. Unsupervised Neural Tracing in Densely Labeled Multispectral Brainbow Images. IEEE 18th International Symposium on Biomedical Imaging (ISBI) 1122–1126 (2021).

The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis

N. S. Michki, Y. Li, K. Sanjasaz, Y. Zhao, F. Shen, L. A. Walker, W. Cao, C.-Y. Lee, and D. Cai. The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis. Cell Reports 35 (4), 109039 (2021).

Teaching Python for Data Science: Collaborative development of a modular & interactive curriculum

M. Duda, K. L. Sovacool, N. Farzaneh, V. Nguyen, S. Haynes, H. Falk, K. L. Furman, L. A. Walker, R. Diao, M. Oneka, A. Drotos, A. Woloshin, G. Dotson, A. Kriebel, L. Meng, S. Thiede, S. Ramdas, Z. Lapp, and B. N. Wolford. Teaching Python for Data Science: Collaborative development of a modular & interactive curriculum Journal of Open Source Education 4(46), 138 (2021).

Robust transcriptional profiling and identification of differentially expressed genes with low input RNA sequencing of adult hippocampal neural stem and progenitor populations.

J. K. Denninger, L. A. Walker, X. Chen, A. Turkoglu, A. Pan, Z. Tapp, S. Senthilvelan, R. Rindani, O. Kokiko-Cochran, R. Bundschuh, P. Yan, E. D. Kirby. Robust transcriptional profiling and identification of differentially expressed genes with low input RNA sequencing of adult hippocampal neural stem and progenitor populations. Frontiers In Molecular Neuroscience 15:810722 (2022).

PP2A is a therapeutically targetable driver of cell fate decisions via a c-Myc/p21 axis in Acute Myeloid Leukemia.

S. Goswami, R. Mani, J. Nunes, C.-L. Chiang, K. Zapolnik, E. Hu, F. Frissora, X. Mo, L. A. Walker, P. Yan, R. Bundschuh, L. Beaver, R. Devine, Y.-T. Tsai, A. Ventura, Z. Xie, Z. Xie, M. Chen, R. Lapalombella, A. Walker, A. Mims, K. Larkin, C. Bennett, M. Phelps, E. Hertleine, G. Behbehani, S. Vasu, J. C. Byrd and N. Muthusamy. PP2A is a therapeutically targetable driver of cell fate decisions via a c-Myc/p21 axis in Acute Myeloid Leukemia. Blood 139(9), 1340–1358 (2022).

nGauge: Integrated and extensible neuron morphology analysis in Python.

L. A. Walker*, J. S. Williams*, Y. Li, D. Roossien, N. S. Michki, and D. Cai. nGauge: Integrated and extensible neuron morphology analysis in Python. Neuroinformatics XXX (2022).

Talks

Tutorial 1 on Relevant Topic in Your Field

March 01, 2013

Tutorial at UC-Berkeley Institute for Testing Science, Berkeley CA, USA

Conference Proceeding talk 3 on Relevant Topic in Your Field

March 01, 2014

Conference proceedings talk at Testing Institute of America 2014 Annual Conference, Los Angeles, CA

Teaching

Java Software Engineering (CSE 2221)

OSU-NIH Summer Research Program

Physical and Chemical Principles Behind Biology and Medicine (Biophysics 370)